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SUMMARY:Phylogenetic hypothesis of the Oleeae tribe (Oleaceae)  Diversif
 ication and molecular evolution patterns in plastid and nuclear ribosomal 
 DNA - Loubab Zedane\, Brockington Lab
DTSTART:20180215T130000Z
DTEND:20180215T133000Z
UID:TALK82701@talks.cam.ac.uk
CONTACT:Pallavi Singh
DESCRIPTION:Because of tremendous variation in anatomy\, floral morphology
 \, and breeding systems as well as its worldwide distribution covering ver
 y different environments\, the Oleeae tribe (Oleaceae) presents a very int
 eresting biological model to study plant diversification and molecular evo
 lution patterns. However\, without a robust phylogenetic framework\, our u
 nderstanding of the mechanisms\, which led to this diversification\, is st
 ill too limited to accurately describe the evolutionary and biogeographic 
 history of this tribe. Reconstruction of the evolutionary history of the O
 leeae tribe and the phylogenetic relationships between its species were th
 us at the core of this work. This was notably addressed by applying a geno
 me skimming strategy in order to assembly complete plastid genomes (mean s
 ize ~155’000 bp) and nearly complete nuclear ribosomal DNA (ETS\, 18S\, 
 ITS1\, 5.8S\, ITS2\, and 26S) (mean size ~7’000 bp) of 74 taxa encompass
 ing all 18 recognized genera of Oleeae. This work has led to significant p
 rogress in resolving the phylogenetic relationships within the tribe at di
 fferent taxonomic levels. We demonstrated that the use of a shotgun approa
 ch is a highly suitable method to generate complete plastomes (ptDNA) and 
 nuclear ribosomal DNA (nrDNA)\, even on herbarium sample. We showed the hi
 gh suitability of the complete plastid genome for constructing a robust an
 d high-resolution phylogeny of the Oleeae tribe and provided new insights 
 into the biogeographic history and the evolution of it breeding system. We
  also demonstrated that nuclear ribosomal DNA structure appears to be infl
 uenced by climatic factors. This latter lie in line with the predictions o
 f the thermostability hypothesis and suggest that this topic needs to be l
 ooked into more in detail for better mechanistic understanding of genome e
 volution.\n
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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