BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Quantitative Super-Resolution Imaging of Biological Samples and DN
 A Nanostructures - Prof. Christian Soeller\, Physics Department\, Universi
 ty of Exeter
DTSTART:20180309T160000Z
DTEND:20180309T170000Z
UID:TALK87121@talks.cam.ac.uk
CONTACT:Lorenzo Di Michele
DESCRIPTION:Quantitative imaging refers to imaging where local image inten
 sities can be directly related to the abundance of markers and\, ideally\,
  targets in biological samples. This has become especially relevant with t
 he advent of molecular resolution imaging in the form of fluorescence supe
 r-resolution microscopy. In this talk I will report on our efforts towards
  achieving truly quantitative and molecular resolution microscopy of ion c
 hannels in single cells. Recent advances have been enabled by progress in 
 several areas\, including improved camera technology\, image analysis and 
 marker approaches. An important role for DNA nanotechnology has arisen fro
 m the ability to create nanostructures that are ideally suited to assessin
 g imaging performance and providing calibration standards. I will illustra
 te how this can be used in routine super-resolution microscopy for quality
  assurance and algorithm testing. Perhaps even more importantly\, the use 
 of synthetic DNA can be used to control the transient binding of dye molec
 ules to markers\, known under acronyms such as DNA-PAINT. This approach ha
 s provided enormous flexibility in designing labelling schemes compatible 
 with high resolution and accurate quantification. These advantages will be
  illustrated with the true molecular scale visualization of variable clust
 ering properties of ryanodine receptors\, a large calcium channel\, in car
 diac myocytes.
LOCATION:Small Lecture Theatre\, Cavendish Laboratory
END:VEVENT
END:VCALENDAR