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SUMMARY:Microfluidic Single-Cell Manipulation and Analysis - Dr. Stefano P
 agliara\, University of Exeter
DTSTART:20171122T103000Z
DTEND:20171122T113000Z
UID:TALK95992@talks.cam.ac.uk
CONTACT:Tom Scheidt
DESCRIPTION:Micro- and nano-scale bioengineering technologies are becoming
  pivotal in biological and health sciences. In particular\, single-cell an
 alysis has recently allowed the discovery of novel antibiotics (1)\, the c
 ultivation of microorganisms from the Human Most Wanted Taxa (2) and an en
 hanced understanding of key biological phenomena such as antibiotic suscep
 tibility in bacteria\, gene expression and stem cell pluripotency (3). \n\
 nIn the first part of my talk I will introduce a novel microfluidic-micros
 copy platform that I have employed to mechanically phenotype live mammalia
 n cells including embryonic stem cells. I will show that the nuclei of som
 e embryonic stem cells display a unique material property that is they are
  auxetic exhibiting a cross-sectional expansion when stretched and a cross
 -sectional contraction when compressed (4). \n\nIn the second part of my t
 alk I will present a microfluidic platform for manipulating and imaging si
 ngle bacteria and microalgae that I have used for investigating single cel
 l response to environmental stress in terms of changes in the expression o
 f selected genes (5).\n\nReferences\n[1] L. L. Ling et al.\, Nature 517\, 
 455 (2015).\n[2] L. Ma et al.\, Proceedings of the National Academy of Sci
 ences 111\, 9768 (2014).\n[3] Y. Wakamoto et al.\, Science 339\, 91 (2013)
 .\n[4] S. Pagliara et al.\, Nature Materials 13\, 638 (2014).\n[5] R. Bamf
 ord et al.\, BMC Biology\, in press.\n
LOCATION:Department of Chemistry\, Cambridge\, Unilever lecture theatre
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