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Visualizing vesicle and virus budding by electron microscopy

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The budding of coated vesicles and membrane viruses can be studied using new electron microscopy methods. I will illustrate how cryo-electron tomography and sub-tomogram averaging can describe the structure and arrangement of protein coats in situ on vesicles and viruses, and how correlative light and electron microscopy can identify intermediates in budding processes. I will present our results from applications of these methods to COPI , clathrin and HIV Gag mediated budding.

This talk is part of the MRC LMB Seminar Series series.

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