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C4 photosynthesis transcriptomics: From Cleome to rice

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C4 photosynthesis involves changes to the biochemistry, cell biology and morphology of leaves. Current estimates indicate that the C4 pathway has evolved at least sixty two times independently in plants. Our understanding of many C4 lineages is limited by the lack of a reference sequence. We used the closest C4 plant to Arabidopsis, Cleome gynandra as a model for C4 photosynthesis. RNA -seq was performed on both Cleome gynandra (C4) and Cleome hassleriana (C3) to profile transcripts of leaves at different stages of development as well as from specific cell. Many changes during evolution from C3 to C4 involved cell specialization between mesophyll and bundle-sheath cells. Candidate genes involved in the development or regulation of C4 photosynthesis including some transcriptional regulators are being currently studied.

This talk is part of the Plant Sciences Research Seminars series.

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