Abstract

The transcriptional regulatory system plays a central role in directing gene expression changes in response to internal and external stimuli. In this talk, first I wll present our work on transcription regulation in yeast, ranging from large-scale experimental studies to computational analyses of regulatory networks. I will introduce results from ChIP-chip experiments that identify genome-wide DNA -binding sites of transcription factors, particularly focusing on the cell cycle regulatory system. I will discuss how these observations fit in to an idea of a genomic regulatory network, and examine the effects of such networks on gene expression. Later, I will introduce preliminary results of our analysis of the transcriptional regulatory system in the human genome.

Vaquerizas JM, Kummerfeld SK, Dopazo J, Teichmann SA & Luscombe NM. A functional census of human transcription factors. (submitted).

Luscombe NM, Babu MM, Yu H, Snyder M, Teichmann SA & Gerstein M. Genomic analysis of regulatory network dynamics. Nature 431, 308-312.

Babu MM, Luscombe NM, Aravind L, Gerstein M & Teichmann SA. Structure and evolution of transcriptional regulatory networks. Current Opinions in Structural Biology, 14, 283-291.

Yu H, Luscombe NM, Qian J & Gerstein M. Genome-wide analysis of gene expression relationships in transcriptional regulatory networks. Trend in Genetics, 19, 422-427.

Horak C E , Luscombe N M , Qian J, Bertone P, Piccirillo S, Gerstein M & Snyder M. (2002). Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae. Genes and Development , 16, 3017-3033.

For further information about our group’s work: http://www.ebi.ac.uk/luscombe