Abstract

Microfluidics is regarded as an attractive technology for (bio)chemical analyses as it opens up the possibility to rapidly perform precisely-controlled high-throughput analyses while consuming only miniature amounts of samples. As part of my PhD I am developing microfluidic devices to quantitatively characterise proteins in their native liquid phase. I will demonstrate our recently published platform for diffusional sizing of proteins (Patent No. EP2912455 A1 ) as well as our developments in microfluidic approaches to determine the charges of biological molecules in liquid phase and compare their performance to existing technologies. I will further demonstrate how microfluidic devices can be used to probe protein-protein interactions in liquid phase as well as to perform high throughput automated protein aggregation assays.