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Essential head genes in the giant PhiKZ-related phages

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  • UserProfessor Julie Thomas, Rochester Institute of Technology, Rochester, United States
  • ClockFriday 16 December 2016, 13:50-14:30
  • HouseOnline.

If you have a question about this talk, please contact Ilana Spilka .

A “giant” phage has a long genome (>200 kb) and a large, structurally complex virion. The head of Salmonella phage SPN3US is composed of about 50 different proteins. Numbers of these proteins, including a multi-subunit RNA polymerase (vRNAP), are packaged inside the head along with the 240 kb dsDNA genome and ejected into the host cell. However, the functions of the majority of head proteins are unknown. In this talk I will discuss how our new collection of SPN3US mutants has facilitated identification of essential head genes and novel features of giant phage head structure, function and assembly.

This talk is part of the Bacteriophage 2017 series.

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