Abstract

Please come along to the monthly campus Single Cell Seminar series. All are welcome, include colleagues from Cambridge (though email me so we can book them in as a visitor with security)

Speaker 1: Qianxin Wu (Cell Ops R&D) ”Targeted single cell transcripts profiling with combinatorial indexing (sci-rna-seq)”

Speaker 2: Valentine Svensson (Teichmann group) ”Bioinformatics Journal Club: ZINB -WaVE”

Qianxin will be giving an update on progress on her work with the sci-rna-seq protocol, which can process thousands of fixed cells in parallel. The bioRxiv manuscript was discussed in a previous single cell seminar, and the protocol has recently been published by the Shendure lab in Science (http://science.sciencemag.org/content/357/6352/661).

Valentine will be presenting a journal club on the ZINB -WaVE manuscript posted on bioRxiv (http://www.biorxiv.org/content/early/2017/04/06/125112). The authors claim that ZINB -WaVE provides a “general and flexible method for signal extraction from single-cell RNA -seq data”, that can handle zero inflation (dropouts), over-dispersion, and the count nature of the data. Valentine will explain some of the underlying concepts, but do look at the paper if you can.